We can see here that after the chemical reaction in glycolysis the six-carbon glucose molecule that split into two three-carbon ions are: D. ATP ⇒ ADP
In glycolysis, the six-carbon glucose molecule is broken down into two three-carbon molecules during the initial steps of the process. The specific chemical reaction where the glucose molecule is split into two three-carbon molecules is called "Glycolysis Step 4: Cleavage of Fructose 1,6-Bisphosphate."
During this step, the enzyme aldolase catalyzes the cleavage of the six-carbon sugar molecule, fructose 1,6-bisphosphate, into two three-carbon molecules: dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P).