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Operational definition for the growth of microorganisms?​

User Daanish
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2 Answers

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Answer:

The bacterial growth can be defined as the increase in the number of the bacterial population by the process of binary fission.

User Matt Davison
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An operational definition for the growth of microorganisms can be:

An increase in the number of viable microorganisms over time observed in a controlled culture environment.

Some key aspects of this definition:

  1. Increase in number: The number of microbial cells or colonies increases quantitatively over multiple generations. Growth leads to a larger population size.
  2. Viable organisms: Only living and metabolically active microbes are counted. Non-viable dead cells are not included.
  3. Over time: Growth is measured by comparing the number of microbes at two or more points in time. It shows a increase in population size over minutes, hours or days.
  4. Controlled culture: The microbes are grown in a controlled laboratory environment with optimized conditions for growth including temperature, nutrients, pH, oxygen level etc. This allows measuring the growth under standard and reproducible conditions.
  5. Observed growth: There is a direct observation and quantification of the increasing number of microbial cells or colonies at different time points. Growth is not inferred but measured empirically.

This operational definition focuses on the measurable and quantifiable increase in viable microbial population size over time in an optimized laboratory culture environment. Please let me know if you need any clarification or have additional questions!

User JeffP
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