Question

Which of the gels could give rise to the hypothetical elution profile below if a sample contains enzyme A (210 kDa); enzyme B (84 kDa); enzyme C (15 kDa); enzyme D (52 kDa); and enzyme E (68 kDa)? Select all that apply.a.1b.2c.3d.4e.5

Answer 1

Enzyme A (210 kDa), enzyme E (68 kDa), and enzyme D (52 kDa) could give rise to the hypothetical elution profile.

Step-by-step explanation:

The hypothetical elution profile indicates the order and relative positions at which the enzymes elute during gel electrophoresis. Based on the given molecular weights of the enzymes, enzyme A (210 kDa), enzyme E (68 kDa), and enzyme D (52 kDa) could give rise to the hypothetical elution profile. This is because the larger enzymes usually elute first, followed by smaller ones due to their different mobilities in the gel matrix. Therefore, the correct options are a) 1, d) 4, and e) 5.