Answer:
ethylene Blue and Bromothymol Blue are commonly used as vital stains in biology and are used to view many types of cells under a light microscope. The use of these substances is a necessary step because they help to increase the contrast between the cells and the surrounding medium, making it easier to visualize the cells and their features.
Methylene Blue and Bromothymol Blue are both basic dyes, which means that they bind to acidic components within the cell such as nucleic acids and proteins, staining them blue or green, respectively. By binding to these components, the dye can highlight the different structures and organelles within the cell, allowing them to be more easily seen under a light microscope.
In addition to increasing contrast, vital stains like Methylene Blue and Bromothymol Blue can also help to distinguish living cells from non-living ones, as the dyes are only taken up by living cells. This makes them useful for identifying and counting cells in a sample.
Overall, the use of vital stains like Methylene Blue and Bromothymol Blue is an important step in the visualization of cells under a light microscope, as they help to improve contrast and highlight cellular structures, allowing researchers to better understand the biology of the cells they are studying.
Step-by-step explanation: