Answer:
Determination of kcat:
Enzyme kinetics refers to the quantitative investigation of enzyme-catalyzed reactions, such as the determination of Michaelis-Menten kinetics parameters, including Vmax, Km, and kcat.
Michaelis-Menten kinetics is a critical aspect of enzyme kinetics that aids in the characterization of enzymes based on their activities. Vmax refers to the maximum initial rate of an enzyme-catalyzed reaction, and Km is the substrate concentration that leads to half the maximum initial velocity.
There are various strategies for determining kcat, which is the enzyme catalytic constant.
It is described as the number of substrate molecules that are converted into product molecules by an enzyme molecule per unit of time when the enzyme is completely saturated with the substrate.
The most direct way of measuring kcat is to use a single turnover method, where the enzyme is initially saturated with the substrate and then mixed with excess enzyme inhibitor or by removing the reaction products as soon as they are formed.
As a result, during the reaction, the enzyme is prevented from engaging in further catalysis.
Enzyme kinetics is an essential topic for life science research as it aids in the characterization of enzymes based on their activities.