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PCR was used to amplify a specific 500-base section of DNA from three birds of the same species that were thought to be related. The three samples of amplified DN

were run on an electrophoresis gel. Three bands were seen on the gel that were exactly the same size. Are the birds related?

2 Answers

4 votes

Final answer:

PCR results showed identical-sized DNA bands from three birds, suggesting identical sequences in the amplified region. This may hint at a relationship, but other genetic analyses are needed for confirmation. PCR is widely used in research and forensics for DNA amplification and further study.

Step-by-step explanation:

Are the Birds Related Based on PCR Results?

The presence of bands of the same size after a Polymerase Chain Reaction (PCR) followed by gel electrophoresis suggests that the amplified 500-base section of DNA from the three birds is the same. This identical size in the DNA fragments could indicate that the birds have identical sequences in the specific region that was amplified, which might suggest a relationship.

However, to conclusively determine if the birds are related, further genetic analyses would be necessary, as this result alone does not confirm relatedness; it only indicates similarity in a specific genetic region. Techniques such as sequencing or other DNA fingerprinting methods such as Restriction Fragment Length Polymorphism (RFLP) analysis might provide additional evidence of a relationship.

The PCR technique utilizes primers, Taq polymerase, and deoxynucleotides to enrich a particular segment of DNA. This targeted approach allows for a minute amount of DNA to be amplified to levels where it can be analyzed. Moreover, PCR amplification of DNA is a common procedure in research and forensic applications due to its ability to generate large quantities of a specific DNA segment, facilitating subsequent studies and analyses, such as determining relationships among individuals or species.

User Viztastic
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Yes, the birds are likely related based on the fact that they all have the same size band on the electrophoresis gel after PCR amplification.

User Avernet
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