Answer: For collecting blood for Bilirubin, Ammonia, PT/PTT, HgB glucose test, and blood cultures, the following tubes should be used and in the following order:
1. Blood Cultures: The first set of blood cultures should be collected in two bottles, one aerobic and one anaerobic, using a sterile technique. The bottles must be labeled with the patient's name, medical record number, date, and time of collection.
2. Light Blue Top Tube: Collect the second tube in a light blue top tube containing sodium citrate as an anticoagulant for PT/PTT testing. The tube should be inverted gently several times to ensure proper mixing of the anticoagulant with the blood.
3. Red Top Tube: Collect the third tube in a red top tube without anticoagulant for Bilirubin, Ammonia, and HgB glucose testing. The tube should be inverted gently several times to ensure proper clotting.
Note: If the patient is on anticoagulant therapy, a different tube with anticoagulant should be used for Bilirubin, Ammonia, and HgB glucose testing.
Make sure that all tubes are properly labeled with the patient's name, medical record number, date, and time of collection. The order of collection is important to avoid contamination or carryover from one tube to another. It is recommended to follow the facility's standard operating procedures for blood collection to ensure accurate and reliable test results.