Final answer:
To generate transgenic mice via homologous recombination, you would need to create a targeting construct containing the gene of interest, introduce it into embryonic stem cells, select for cells with the desired gene insertion, inject the modified cells into mouse embryos, and screen the offspring for gene insertion. This process is complex and requires specialized techniques and equipment.
Step-by-step explanation:
To generate transgenic mice via homologous recombination, you would need to follow these steps:
Create a targeting construct: This involves designing a piece of DNA that contains the gene of interest and specific DNA sequences that are homologous to the target site in the mouse genome.
Introduce the targeting construct into embryonic stem cells (ES cells): These are derived from early-stage mouse embryos and have the ability to differentiate into various cell types.
Select for cells that have undergone homologous recombination: This is done by using antibiotics or other selection markers that are linked to the targeting construct.
Inject the modified ES cells into mouse embryos: The modified ES cells will contribute to the development of the mouse offspring, leading to the generation of transgenic mice.
Screen the offspring for gene insertion: This can be done using PCR or other molecular techniques to confirm the presence of the gene of interest in the mouse genome.
It is important to note that generating transgenic mice via homologous recombination is a complex process that requires specialized techniques and equipment.