Final answer:
The removal of the last primer leaves a gap that cannot be primed at the ends of the chromosome on both the leading and lagging strands of DNA replication.
Step-by-step explanation:
On the lagging strand of DNA replication, the DNA is synthesized in short fragments called Okazaki fragments. Each of these fragments requires a new primer for DNA synthesis to begin. When the last primer is removed, it leaves a gap that cannot be primed at the end of the chromosome. This happens because there is no available template for DNA synthesis at the very end of the lagging strand. Therefore, the removal of the last primer on the lagging strand leaves a gap that cannot be primed at the ends of the chromosome.