Final answer:
False, ELISA tests typically use an enzyme-linked mechanism that leads to a colored product through a chromogenic substrate, and although some ELISAs may involve a fluorogenic substrate that fluoresces after enzyme activation, they typically do not use a fluorescent dye as standard.
Step-by-step explanation:
While immunofluorescence assays do use fluorescent dyes to label and trace antibodies, ELISA tests typically use an enzyme-linked immunosorbent assay. In ELISA tests, secondary antibodies are conjugated to an enzyme rather than a fluorescent dye. When a substrate, often chromogenic, is added, it is converted by the enzyme to a colored product if the antigen-antibody reaction is present, which can be quantitatively measured through spectrophotometry. Some ELISA tests may use a fluorogenic substrate that becomes fluorescent upon enzyme activation, but it is not common to describe them as using a fluorescent dye.