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Because mycoplasmas lack peptidoglycan, their Gram stain result would be:

A)Gram-positive
B)Gram-negative
C)None of these
D)Acid-fast

1 Answer

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Final answer:

Mycoplasmas would yield a Gram-negative result in the Gram stain procedure because they lack peptidoglycan in their cell wall.

Step-by-step explanation:

Because mycoplasmas lack peptidoglycan, their Gram stain result would be: B)Gram-negative. The Gram staining method is used to differentiate bacteria based on their reaction to the staining procedure. Gram-positive bacteria have a thick cell wall composed mainly of peptidoglycan, while Gram-negative bacteria have a thin cell wall and an outer membrane. Mycoplasmas, which are a type of bacteria, do not have a cell wall made of peptidoglycan, so they would not retain the crystal violet stain and would appear Gram-negative. Mycoplasmas, unique among bacteria, lack a cell wall or possess a minimal cell wall without peptidoglycan. In the context of the Gram stain, a widely used technique for bacterial classification based on cell wall characteristics, mycoplasmas present an anomalous case. Since the Gram stain relies on the ability of cells to retain crystal violet dye within the peptidoglycan layer, mycoplasmas do not fit the conventional Gram-positive or Gram-negative categorization. Therefore, the correct answer is: C) None of these Mycoplasmas don't exhibit the typical Gram staining characteristics due to the absence of peptidoglycan in their cell walls. They are an exception to the Gram classification system.

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