Answer If O, P, and Q are collinear points with PQ = 3, and P' and Q' are the images of P and Q under dilation from O with a scale factor of 4, then the length of the segment P'Q' will be 4 times the length of the segment PQ. Since PQ = 3, the length of P'Q' will be 4 * 3 = $\boxed{12}$.
To see why this is the case, recall that a dilation with a scale factor of 4 expands or contracts a figure by a factor of 4 in each direction. In this case, the dilation from O with a scale factor of 4 expands the segment PQ by a factor of 4, resulting in the segment P'Q' with a length of 4 times the length of PQ. Since PQ = 3, the length of P'Q' will be 4 * 3 = 12.