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How to differentiate between primary and secondary follicle?

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Final answer:

Primary follicles start with a single layer of granulosa cells, but the granulosa cells then become active and transition from a flat or squamous shape to a rounded, cuboidal shape as they increase in size and proliferate. As the granulosa cells divide, the follicles-now called secondary follicles-increase in diameter, adding a new outer layer of connective tissue, blood vessels, and theca cells-cells that work with the granulosa cells to produce estrogens.

Step-by-step explanation:

After puberty, a few primordial follicles will respond to a recruitment signal each day, and will join a pool of immature growing follicles called primary follicles. Primary follicles start with a single layer of granulosa cells, but the granulosa cells then become active and transition from a flat or squamous shape to a rounded, cuboidal shape as they increase in size and proliferate.

As the granulosa cells divide, the follicles-now called secondary follicles-increase in diameter, adding a new outer layer of connective tissue, blood vessels, and theca cells-cells that work with the granulosa cells to produce estrogens.

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