Final answer:
The second attempt of British colonization occurred in Roanoke Island, but the colony was abandoned, and the colonists were never heard from again.
Step-by-step explanation:
The second attempt of British colonization in North America occurred in the late sixteenth century with the establishment of a colony in Roanoke Island, North Carolina, by John White and a group of English colonizers. However, White had to return to England for additional support and was unable to return for several years due to the Spanish Armada and British naval mobilization. When he eventually returned, he found the colony abandoned with the word 'Croatoan' carved into a tree, suggesting the colonists may have fled to a nearby island. The English colonists were never heard from again, and no permanent English colony in North America was established until after Queen Elizabeth's death in 1603.