Final answer:
The organisms used in the Starch Hydrolysis Test include Staphylococcus aureus, S. saprophyticus, Escherichia coli, and Pseudomonas aeruginosa to determine their starch-degrading capabilities.
Step-by-step explanation:
In the Starch Hydrolysis Test, a variety of bacterial organisms were used. Notably, overnight TSA plate cultures of Staphylococcus aureus, S. saprophyticus, Escherichia coli, and Pseudomonas aeruginosa were utilized to assess their ability to hydrolyze starch.
The test aims to identify bacteria that can secrete enzymes like amylase, which break down starch into smaller units such as dextrins, maltose, and ultimately glucose, for absorption by cells. Natural starch, being a mixture of two polymers, amylose and amylopectin, is broken down sequentially by these enzymes.
The characteristic blue-violet color that appears when starch is treated with iodine signifies a positive reaction, indicating the presence of starch in the medium.