Final answer:
At an alkaline pH in hemoglobin electrophoresis, hemoglobin F cannot be separated from hemoglobin S.
Step-by-step explanation:
In hemoglobin electrophoresis at an alkaline pH, the hemoglobin molecules migrate towards anode (+ve) based on their charge. At pH 8.6, all plasma proteins have a negative charge. Among the given options, Hb F (fetal hemoglobin) cannot be separated from Hb S (sickle cell hemoglobin) because they have similar charges and move together during electrophoresis.