Question

In a laboratory setting, a diffusion method involves using filter paper discs impregnated with an antibiotic on a Mueller Hinton agar plate covered with organisms. After 12-24 hours of growth, the zone of inhibition is observed. What is the purpose of examining the zone of inhibition in this procedure?

Answer 1

The purpose of examining the zone of inhibition in the diffusion method is to determine the effectiveness of the antibiotic against the organisms being tested.

Step-by-step explanation:

The purpose of examining the zone of inhibition in the diffusion method is to determine the effectiveness of the antibiotic against the organisms being tested. The zone of inhibition is a clear area around the filter paper disc where the growth of the organisms has been inhibited by the antibiotic. By measuring the diameter of the zone, one can assess the effectiveness of the antibiotic in inhibiting the growth of the organisms.