Final answer:
Failure to obtain blood during venipuncture can be caused by a lost vacuum seal in the tube or an injury to the blood vessel.
Step-by-step explanation:
Failure to obtain blood during venipuncture can be caused by various reasons. One possibility is that the tube has lost its vacuum seal due to a manufacturing defect, an expired tube, or a crack in the tube. When the vacuum seal is compromised, blood may not be drawn properly into the tube. Another possible reason for failure is an injury to the blood vessel, resulting in blood and blood components leaking out.