Final answer:
The 8 steps in vesicle targeting and fusion are vesicle formation, vesicle transport, tethering, docking, priming, membrane fusion, content release, and membrane recycling.
Step-by-step explanation:
The 8 steps in vesicle targeting and fusion are as follows:
- Vesicle formation: Transport vesicles are formed from the Golgi apparatus or endoplasmic reticulum.
- Vesicle transport: The vesicles travel to their target location within the cell.
- Tethering: The vesicle is tethered to the target membrane by protein-protein interactions.
- Docking: Specific proteins on the vesicle membrane interact with proteins on the target membrane, bringing them into close proximity.
- Priming: Additional proteins and molecules are recruited to prepare the vesicle and target membrane for fusion.
- Membrane fusion: The membranes of the vesicle and target membrane merge together, allowing the contents of the vesicle to be released into the target membrane.
- Content release: The cargo molecules in the vesicle are released into the target membrane.
- Membrane recycling: After fusion, the vesicle membrane is retrieved and recycled for future use.