Question

*capsule stain* procedure (6 steps)

Answer 1

The capsule stain procedure is a staining technique used to visualize bacterial capsules using negative staining.

Step-by-step explanation:

The capsule stain procedure is a staining technique used to visualize bacterial capsules, which are protective outer structures. Capsules are best visualized using negative staining, where a dye is used to stain the background but does not penetrate the capsules. The capsules appear as clear layers around the bacterial cells. Here are the steps for the capsule stain procedure:

1. Mix a culture of bacteria with a capsule stain, such as India ink or nigrosin.
2. Place a drop of the mixture on a glass slide.
3. Spread the drop across the surface of the slide to create a thin film.
4. Allow the slide to air dry.
5. Do not heat-fix the specimen.
6. Observe the slide under a microscope using the oil immersion objective lens.