Final answer:
The direction of synthesis of an RNA transcript is from the 5' end to the 3' end.
Step-by-step explanation:
The direction of synthesis of an RNA transcript is 5' —> 3'.
During transcription, RNA polymerase reads the DNA template in a 3' to 5' direction and synthesizes the RNA molecule in a 5' to 3' direction. This means that the RNA polymerase moves along the DNA template from the 3' end to the 5' end.
For example, if the DNA template strand is 3'-CTAGGCTA-5', the synthesized RNA molecule will be 5'-GAUCCGAU-3'.