Final answer:
Alfred Hershey and Martha Chase conducted an experiment using a bacteriophage to determine that DNA, and not proteins, is the genetic material.
Step-by-step explanation:
The investigators responsible for the discovery that DNA, and not proteins, was the genetic material are Alfred Hershey and Martha Chase. They conducted an experiment using a bacteriophage, a virus that infects bacteria, to determine the role of DNA and proteins in genetic transmission. By labeling the phage DNA with radioactive phosphorus (32P) and the protein coat with radioactive sulfur (35S), they were able to show that only the DNA, but not the protein, entered the bacterial cells during infection. This led them to conclude that DNA is the genetic material.