Final answer:
The Tiber and the Po are the two major rivers that may affect transportation and the growth of Italian cities.
Step-by-step explanation:
The two major rivers that may affect transportation and the growth of Italian cities are the Tiber and the Po.
Rivers have historically played a vital role in the growth of cities as they provide fresh water for drinking and irrigation, and were the most efficient way to transport cargo before the age of trains and highways. Navigable rivers often create break-of-bulk points where cities form.
In the case of Italy, the two major rivers that have had significant impacts on transportation and the growth of Italian cities are the Tiber and the Po.