Final answer:
During metaphase, chromosomes line up at the cell's equator, and spindle fibers move them there. This alignment ensures their proper separation into daughter cells.
Step-by-step explanation:
During metaphase, the correct answer to where chromosomes line up and what moves them there is A) Chromosomes line up at the cell's equator, and spindle fibers move them there. These spindle fibers attach to the centromere of each pair of sister chromatids.
The sister chromatids line up at the equator, or center, of the cell, also known as the metaphase plate. The engagement of spindle microtubules to the kinetochores of chromosomes ensures they are positioned correctly for subsequent separation and distribution to daughter cells.