Final answer:
Sodium azide inhibits the electron transport chain in respiration, leading to a decrease in oxygen uptake and ethanol production.
Step-by-step explanation:
Sodium azide inhibits the electron transport chain in respiration. If added after 4 hours to the investigation, it would affect oxygen uptake and the production of ethanol in the following way:
Oxygen uptake would decrease due to the inhibition of the electron transport chain. Sodium azide blocks the transfer of electrons, so the normal flow of electrons and the reduction of oxygen to water would be disrupted. As a result, less oxygen would be consumed.
Similarly, ethanol production would decrease. The electron transport chain is necessary for the production of ATP, which is required for glycolysis and the subsequent conversion of pyruvate to ethanol. Without the normal functioning of the electron transport chain, ATP production would be reduced, leading to a decrease in ethanol production.