Question

What are factors that can affect staining results in microscopy: 1. Insufficient stain time in blue/purple stain or excessive time in red stain. 2. excessive rinsing

3. Blue stain is too dilute or acidic. 4. All of the above

Answer 1

Several factors can affect staining results in microscopy, including insufficient stain time, excessive rinsing, and using a dilute or acidic stain.

Step-by-step explanation:

There are several factors that can affect staining results in microscopy:

1. 
   
3. Insufficient stain time in blue/purple stain or excessive time in red stain: If the stain is not applied for the correct amount of time, the cells may not be properly stained and may appear faint or unclear.
4. 
   
6. Excessive rinsing: If the slide is rinsed too much after staining, the dye may be washed away, resulting in faded or incomplete staining.
7. 
   
9. Blue stain is too dilute or acidic: If the blue stain used is not concentrated enough or if it is too acidic, it may not effectively bind to the cells, leading to poor staining.
10. 
    
12. All of the above: All of the factors mentioned above can affect staining results in microscopy.