Final answer:
To determine the specific type of lymphoma, a lymph node biopsy is performed, and the tissue is examined for abnormal cells and antigens using techniques such as immunohistochemistry. During the biopsy, maintaining sterile technique and using appropriate protective gear is crucial, and specimens must be correctly labeled and promptly sent to the laboratory.
Step-by-step explanation:
Physicians determine the specific type of lymphoma, such as Hodgkin's disease, by conducting a lymph node biopsy. The tissue obtained from the biopsy is examined under a microscope, and tests such as immunohistochemistry are performed to observe the presence of abnormal cell size or shape and to identify specific cell-surface antigens that are characteristic of different subtypes of lymphoma. During the biopsy, it is critical to maintain sterile technique to prevent infection or specimen contamination.
The use of mask, gloves, and gown by assisting personnel is also essential to ensure safety and aseptic conditions. Once the specimen is collected, it should be correctly labeled with the patient's name and other details before being promptly sent to the laboratory for histologic examination. All instruments used should be placed in a sealed and labeled container following local protocols for the management of potentially contaminated materials.