Final answer:
The timing for an autoclave load should begin when the proper pressure and the desired temperature are reached. The conditions for sterilization in an autoclave include temperatures typically at 121ºC or 132ºC and pressure of 15 to 20 psi.
Step-by-step explanation:
The timing of an autoclave load should begin when the proper pressure is reached and the desired temperature is reached. Autoclave operation involves raising the temperature of steam above the boiling point of water under increased pressure to achieve sterilization. This process is crucial for killing endospores and ensuring the complete sterility of items such as surgical instruments.
Standard operating temperatures for autoclaves are typically 121ºC or 132ºC, with pressures around 15 to 20 psi. The items being sterilized must be directly exposed to this steam, which entails having containers loosely closed and instruments loosely wrapped. Quality control measures, including the use of indicators such as autoclave tape and biological spore tests, are employed to confirm that proper sterilization temperatures and conditions have been met.