Question

Each scenario below describes an operational error that can lead to inaccurate or irreproducible TLC results. In each case describe the effect on the TLC results and briefly explain why.

Spots were applied to a TLC plate so that they were exactly even with the solvent level in the developing chamber.

Answer 1

Operational errors in TLC can lead to inaccurate or irreproducible results. Spots applied exactly even with the solvent level can cause unequal separation. Using a concentrated staining agent can lead to heavily concentrated regions on the TLC plate.

Step-by-step explanation:

The operational errors that can lead to inaccurate or irreproducible TLC (thin-layer chromatography) results include:

1. Spots were applied to a TLC plate so that they were exactly even with the solvent level in the developing chamber. This can lead to inaccurate results because if the spots are exactly even with the solvent level, they may not travel up the TLC plate at the same rate as other spots. This can result in unequal separation and inaccurate measurement.
2. Using a staining agent that is too concentrated can lead to heavily concentrated regions on the TLC plate. This can make it difficult to accurately measure the distance traveled by each spot and determine the relative component concentrations.

It is important to carefully follow the proper procedures and use appropriate controls to ensure accurate and reproducible TLC results.