Question

Each scenario below describes an operational error that can lead to inaccurate or irreproducible TLC results. In each case describe the effect on the TLC results and briefly explain why.

A lab worker frequently removed the developing chamber cover in order to better see the position of the solvent front during development of the plate.

Answer 1

Frequently removing the developing chamber cover during TLC development can lead to inaccurate or irreproducible TLC results due to introduction of contaminants and uneven solvent evaporation.

Step-by-step explanation:

The effect of frequently removing the developing chamber cover during TLC development is that it can lead to inaccurate or irreproducible TLC results. This is because removing the cover allows for exposure to air, which can introduce contaminants onto the TLC plate. Contaminants can interfere with the separation of the compounds on the plate, leading to inaccurate results. Additionally, removing the cover can lead to uneven solvent evaporation, which can result in uneven development of the plate and inaccurate measurement of the solvent front position.