Question

Each scenario below describes an operational error that can lead to inaccurate or irreproducible TLC results. In each case describe the effect on the TLC results and briefly explain why.

Samples were applied to a TLC plate in spots that were 6 mm in diameter.

Answer 1

An operational error that can lead to inaccurate TLC results is applying samples that are too large or too small to spots that are 6 mm in diameter.

Step-by-step explanation:

An operational error that can lead to inaccurate or irreproducible TLC results is applying samples that are too large to spots that are 6 mm in diameter. This can result in the spots overlapping or spreading too much, making it difficult to accurately analyze the separation on the TLC plate. Similarly, applying samples that are too small can also lead to inaccurate results because the spots may be too faint or not visible, making it difficult to measure the separation.