Question

The human CFTR gene has 27 exons. To clone the human CFTR gene into a plasmid vector for expression in bacteria, one could______

A. Use restriction enzymes to cut out the CFTR gene from human DNA and ligate it into the plasmid vector
B. Use reverse transcriptase to make a cDNA from human cell mRNA, and then ligate adaptors containing restriction enzyme sites for cloning into the plasmid vector
C. Use PCR to copy to CFTR gene from human DNA, and then ligate adaptors containing restriction enzyme sites to clone into the plasmid vector

Answer 1

The correct method to clone the human CFTR gene into a plasmid vector for expression in bacteria is to use reverse transcriptase to make a cDNA from human cell mRNA and then ligate adaptors containing restriction enzyme sites for cloning into the plasmid vector. The correct answer is option B )

Step-by-step explanation:

The correct answer is option B. Use reverse transcriptase to make a cDNA from human cell mRNA, and then ligate adaptors containing restriction enzyme sites for cloning into the plasmid vector.

When cloning the human CFTR gene into a plasmid vector for expression in bacteria, using reverse transcriptase to make a cDNA from human cell mRNA is the appropriate method. Reverse transcriptase is used to convert mRNA into cDNA, which can then be ligated with adaptors containing restriction enzyme sites for cloning into the plasmid vector.

This method allows for the insertion of the CFTR gene into the plasmid vector in a way that is compatible with the bacterial host and facilitates further study of the gene.