Final answer:
Calibrating an optical microscope involves securely placing the microscope on a lab table, starting with the lowest power objective, focusing the specimen, adjusting the condenser lens and light settings for clarity, moving to higher magnifications, and ensuring the ocular lenses are properly distanced. Proper calibration ensures an accurate and clear viewing experience.
Step-by-step explanation:
Steps for Calibrating an Optical Microscope
To ensure accurate and clear observations when using an optical microscope, it is essential to calibrate it correctly. The calibration process begins by carrying the microscope carefully with two hands to your lab table and setting it down gently to avoid shocking the optical system. Once securely placed, begin the calibration as follows:
Secure the specimen onto the stage with the slide clips and position it over the light source using the x-y mechanical stage knobs.
Start with the lowest power objective lens and use the course focusing knob to get a rough focus on the specimen.
Adjust the condenser lens and iris diaphragm to achieve optimal contrast and clarity in the image.
For higher magnification, switch to a higher power objective lens, then use the fine focus knob to refine the image, and adjust the light intensity, condenser, and iris diaphragm as necessary for a bright and sharp image.
Check the distance between the ocular lenses to ensure it matches the interpupillary distance of your eyes for a comfortable viewing experience.
At the close of your lab session, remember to return the microscope to its cabinet with the ocular lenses facing in to protect them. This careful handling and calibration process will allow for the most effective use of the optical microscope.