Question

you are trying to determine a tlc solvent system which will separate compounds a, b, and c.you ran the compounds on a tlc plate using hexanes/ethyl acetate 95:5 as the eluding solvent and obtained the following chromatogram: how could you change the solvent system to give better separation of these three compounds?

Answer 1

Thin layer chromatography is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing and a mobile phase. The separation principle is in the different affinities between the components of the mixture and the stationary or mobile phase.

The affinity in mobile phase could be improved changing the polarity of this phase. In this case, you could change proportion of hexane/ethyl acetate to change polarity of mobile phase and the affinity of the different compounds to mobile or stationary phase.

Step-by-step explanation: