Question

A similar protein is found in both a bacterial cell and a single-celled eukaryote. However, the prokaryote seems to produce a much higher amount of the protein. A detailed investigation of the control mechanisms of this protein in both organisms would include which of the following experiments?

a) Comparative genomics analysis
b) Western blotting
c) PCR amplification
d) Electrophoresis

Answer 1

A detailed investigation of the control mechanisms of a protein in both a bacterial cell and a single-celled eukaryote would include comparative genomics analysis, western blotting, and PCR amplification.

Step-by-step explanation:

The detailed investigation of the control mechanisms of a protein in both a bacterial cell and a single-celled eukaryote would include the following experiments:

1. Comparative genomics analysis: This experiment compares the DNA sequences of the protein-coding genes in both organisms to identify similarities and differences in their regulation.
2. Western blotting: This experiment detects and quantifies the amount of the protein produced in each organism, allowing for a comparison of protein levels.
3. PCR amplification: This experiment amplifies the DNA sequence of the protein-coding gene in both organisms, providing enough material for further analysis.