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Two ATP molecules are used to initiate glycolysis. Enzymes generate molecules of ATP for each molecule of glucose that undergoes glycolysis. Thus a net gain of _________ molecules of ATP is produced in glycolysis.

User Anusreemn
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Final answer:

In glycolysis, there is a net gain of two ATP molecules produced.

Step-by-step explanation:

Glycolysis starts with glucose and ends with two pyruvate molecules, a total of four ATP molecules and two molecules of NADH. Two ATP molecules were used in the first half of the pathway to prepare the six-carbon ring for cleavage, so the cell has a net gain of two ATP molecules and 2 NADH molecules for its use. If the cell cannot catabolize the pyruvate molecules further, it will harvest only two ATP molecules from one molecule of glucose.

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