Final answer:
Jay's Treaty led to Spain negotiating Pinckney's Treaty, which allowed American commerce to flow through New Orleans.
Step-by-step explanation:
Jay's Treaty had a significant effect on the relationship between the U.S. and Spain. The treaty between the U.S. and Britain, Jay's Treaty, led Spain to negotiate Pinckney's Treaty with the U.S. Spain was afraid that an alliance between the U.S. and Britain would threaten their control over their New World Empire. As a result, Pinckney's Treaty allowed American commerce to flow through the Spanish port of New Orleans, benefiting American farmers in the Ohio River Valley.