Final answer:
Automatic rescaling is the process when histogram pixel values are manipulated, particularly relevant in flow cytometry and X-ray crystallography to display fluorescence intensity or X-ray interference patterns.
Step-by-step explanation:
When the pixel values of an image are manipulated by its histogram, this process is commonly referred to as Automatic Rescaling. In the context of flow cytometry, histograms play a crucial role. A histogram can depict data collected from forward- and side-scatter detectors with forward scatter on the y-axis and side scatter on the x-axis, where the latter is presented on a logarithmic scale to represent fluorescence intensity.
For instance, a histogram in flow cytometry might show two peaks: one indicating cells without fluorescence, not expressing a particular marker like CD8 due to the absence of an antibody bond; the other indicating high fluorescence readings where cells are bound by the antibody, expressing CD8. In addition, when discussing the manipulation of pixel values in the context of X-ray crystallography, the increase or decrease in amplitude (intensity) due to interference of X-rays can be visualized and analyzed via histograms.