Question

You are in charge of engineering a new cell line for use in an industrial-scale production process. The cell line must be eukaryotic and able to survive solely utilizing glucose for energy. Glucose will be constantly present to keep the cells functioning. The cell line also needs to be extremely energy efficient, consuming significant energy and turning it into viable output.

What type of promoter would you use for expression of glucose-6-phosphatase in this engineered cell line?

Answer 1

A glucose-responsive promoter should be used for the expression of glucose-6-phosphatase in the engineered cell line to regulate its expression based on the availability of glucose as an energy source.

Step-by-step explanation:

The type of promoter that should be used for the expression of glucose-6-phosphatase in this engineered cell line is a promoter that is specific to glucose metabolism. One example of such a promoter is the glucose-responsive promoter, which is activated in the presence of glucose. This type of promoter would ensure that the expression of glucose-6-phosphatase is tightly regulated and only occurs when glucose is available as a energy source.