Final answer:
Biological spore tests using Geobacillus stearothermophilus spores should be conducted on autoclaves at least weekly for routine monitoring, but frequency may increase based on the volume of use or specific accreditation requirements within healthcare and laboratory settings.
Step-by-step explanation:
The autoclave is a critical device for ensuring the sterility of medical instruments and laboratory equipment. The standard operating procedures for autoclaves involve reaching temperatures of 121 °C or 132 °C at pressures of 15 to 20 pounds per square inch (psi).
To ensure that an autoclave is functioning correctly and achieving the necessary conditions to kill even the most heat-resistant bacterial endospores, it is essential to perform regular quality control tests.
One of the most reliable methods for testing the effectiveness of autoclaves is through the use of biological spore indicators, particularly using Geobacillus stearothermophilus spores. The autoclave must demonstrate its ability to kill these spores to assure proper sterilization.
For routine monitoring of autoclaves, the Centers for Disease Control and Prevention (CDC) recommends performing biological spore tests at least weekly. However, many healthcare facilities and laboratories choose to conduct these tests more frequently based on factors like the volume of autoclaving or adherence to specific accreditation standards.