Final answer:
The correct incubation temperature range for a biological indicator after use in a steam sterilizer is typically 55-65°C, for cultivating potential surviving spores and validating the sterilization process. The autoclave operates at higher temperatures of 121°C or 132°C; however, incubation occurs at a lower range to encourage growth of any resilient microorganisms for detection.
Step-by-step explanation:
The correct incubation temperature range for a biological indicator used in a steam sterilizer is typically around 55-65°C for mesophilic organisms, rather than 95-105°C. These indicators contain spores of highly resistant microorganisms such as Geobacillus stearothermophilus, an obligate thermophile that can withstand high temperatures. However, the standard operating temperatures for autoclaves that achieve sterilization are usually 121°C or 132°C at a pressure of 15 to 20 psi.
After a sterilization cycle, biological indicators must be incubated to ensure that the endospores have been effectively killed. The required incubation conditions for biological indicators are typically lower than the sterilization temperature to allow for the growth of any surviving microorganisms, hence the suggested range of 55-65°C. Standard method calls for incubation at 35°C ± 2°C and this might be modified as per lab schedules. The quality control process ensures that autoclaving has achieved the necessary conditions for sterilization.