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Which of the following dyes are used in Sternheimer-Malbin stain?

A. Hematoxylin and eosin
B. Crystal violet and safranin
C. Methylene blue and eosin
D. Methylene blue and safranin

User Januw A
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1 Answer

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Final answer:

The Sternheimer-Malbin stain uses crystal violet and safranin, which are basic stains that bind to the negatively charged cell walls due to their positive chromophores.

Step-by-step explanation:

The Sternheimer-Malbin stain utilized in microscopy for highlighting cells in urinary sediment consists of two specific dyes which are crystal violet and safranin. These dyes are basic stains, and because cells typically have negatively charged cell walls, the positive chromophores in these dyes are attracted to the cell walls. Therefore, the correct answer to which dyes are used in Sternheimer-Malbin stain is B. Crystal violet and safranin.

User FatDaemon
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