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Which description of sediment with Sternheimer-Malbin stain is correct?

A. Transitional epithelium: cytoplasm pale blue, nucleus dark blue
B. Renal epithelium: cytoplasm light blue, nucleus dark purple
C. Glitter cells: cytoplasm dark blue, nucleus dark purple
D. Squamous epithelium: cytoplasm pink, nucleus pale blue

1 Answer

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Final answer:

The appropriate description of sediment with Sternheimer-Malbin stain is for Transitional epithelium, with the cytoplasm being pale blue and the nucleus being dark blue.

Step-by-step explanation:

The correct description of sediment with Sternheimer-Malbin stain is Transitional epithelium: cytoplasm pale blue, nucleus dark blue. Sternheimer-Malbin stain is widely used in cytology and for staining urinary sediments. In the staining process, nuclei generally stain dark blue or purple, whereas the cytoplasm may take on a variety of shades including pale blue, due to differences in the composition of the cellular material and the specific dye uptake of different cell types.

For example, in transitional epithelium, found in the bladder, the cytoplasm stains a pale blue with the nucleus appearing dark blue when a Sternheimer-Malbin stain is applied. This is consistent with the general principle that acidic cellular components, like nucleic acids in the nucleus, tend to stain darker with basic dyes like those found in Sternheimer-Malbin stain.

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