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When collecting blood to preform a manual WBC differential which blood tube type is preferred?

A. Blue
B. Lavender
C. Grey
D. Green

User Yodi
by
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2 Answers

5 votes

Final answer:

The preferred tube for collecting blood for a manual WBC differential is the lavender tube, which contains the anticoagulant EDTA to preserve blood cells for accurate analysis.

Step-by-step explanation:

When collecting blood to perform a manual WBC (white blood cell) differential, the preferred type of blood tube is B. Lavender. This type of tube contains EDTA (ethylene diamine tetraacetic acid), which is an anticoagulant that preserves the shape of cells and prevents clotting. This is crucial for accurate manual WBC differentials, as the blood cells need to be intact and freely suspended in plasma.

User Localhousee
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7.5k points
6 votes

Final Answer:

When collecting blood to preform a manual WBC differential B. Lavender blood tube type is preferred. So, Option B is correct.

Step-by-step explanation:

In the realm of blood collection for a manual White Blood Cell (WBC) differential, the preferred blood tube type is Lavender. Lavender-top tubes are traditionally associated with ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. EDTA helps prevent blood clotting by binding calcium ions, preserving the blood sample in a state representative of its in vivo condition. This is crucial for accurate WBC differentials, where the goal is to analyze the various types of white blood cells present in the sample.

The selection of Lavender tubes over other options aligns with the need for an anticoagulant that maintains cell integrity and morphology. Blue-top tubes, often containing citrate, may alter cell structure due to calcium chelation, rendering them less suitable for WBC differentials. Grey-top tubes, containing fluoride and oxalate, are more geared towards glucose testing, making them less ideal for WBC differentials.

Green-top tubes, containing heparin, may interfere with staining procedures, affecting the accuracy of WBC differential results. Lavender-top tubes strike a balance, providing the necessary anticoagulation without introducing significant artifacts that could compromise the analysis.

In summary, the preference for Lavender tubes in collecting blood for manual WBC differentials is grounded in the need for an anticoagulant that preserves cell morphology and minimizes interference with subsequent staining procedures, ensuring accurate and reliable results in the assessment of white blood cell types. So, Option B is correct.

User BnJ
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8.4k points