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Processing of skin that is to be frozen shall be initiated within ____days of retrieval, provided the skin is placed in tissue storage media which is replaced at least every___hours.

a. 14, 96
b. 14,72
c. 10,96
d. 10,72

1 Answer

3 votes

Final answer:

The correct processing time for skin to be frozen is initiation within 24 hours of retrieval, and the storage media should be replaced every 24 hours to maintain tissue viability for long-term storage.

Step-by-step explanation:

The processing of skin that is to be frozen shall be initiated within 24 hours of retrieval, and it must be placed in tissue storage media, which should be replaced at least every 24 hours. This protocol ensures the long-term storage viability of the skin tissue and maintains its short-term temperature stability as well as post-preparative stability. This question is about tissue preservation practices, specifically regarding skin grafts or donations that are kept at ultra-low temperatures to ensure cell viability before transplantation or use in medical treatments.

Skin tissues stored in this manner may undergo a freeze-thaw cycle as part of the processing. The slow reduction in temperature and subsequent storage in liquid nitrogen at -196°C are critical steps to protect the tissues from ice crystal formation that can damage cell structures. This careful control of the freezing process, combined with the regular replacement of storage media, is essential for maintaining tissue integrity during long-term storage.

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