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What is the correct temperature for short-term storage frozen tissue?

a. -30 degree celsius or colder
b. -4 degree celsius or colder
c. -20 degree celsius or colder
d. -40 degree celsius or colder

User Milbrandt
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Final answer:

Option C. The correct temperature for short-term storage of frozen tissue is -20 degrees Celsius or colder to maintain sample integrity and suppress microbial activity.

Step-by-step explanation:

The correct temperature for short-term storage of frozen tissue is -20 degrees Celsius or colder. While refrigeration temperatures between 0 °C and 7 °C can inhibit microbial metabolism and slow growth, the preservation of tissue samples requires colder settings to ensure cellular structures remain intact and microbial activity is suppressed. For ultra-low, long-term storage, temperatures around -196 °C in liquid nitrogen tanks or -70 °C in ultra-low freezers are used. However, for short-term usage, maintaining a temperature of -20 °C is generally sufficient for sample integrity during storage and before further analysis or use.

The correct temperature for short-term storage of frozen tissue is -20 degree Celsius or colder. Storing tissue at this temperature helps to maintain its post-preparative stability and prevents any damage from freeze-thaw cycles. Bacterial cultures and medical specimens requiring long-term storage are often frozen at even lower temperatures to ensure their stability.

User Davidsbro
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