Final answer:
When closing the line, all surfaces, including benches, equipment, and the air conditioning system, need to be cleaned and sanitized. PPE should be worn during cleaning, and contaminated waste must be properly disposed of through incineration or autoclaving after treatment. Regular maintenance of systems such as air conditioning is also required to prevent biofilm growth.
Step-by-step explanation:
When closing the line in a clinical or laboratory setting, it is crucial to clean and sanitize all surfaces that have been in contact with any specimens, equipment, or have had exposure to infectious agents. This includes work benches, equipment surfaces, containers, as well as the air conditioning systems that may harbor infectious biofilms. Surfaces must be cleaned with detergent, followed by a disinfectant such as a 1 N sodium hydroxide (NaOH) solution or a 1:10 dilution of bleach, ensuring that the areas are free of pathogens before the next workday begins. Personal protective equipment (PPE) like protective coats, face protection, and cut-resistant gloves must be worn during cleaning to prevent contact with infectious materials. Once the physical cleaning is completed, items like contaminated waste must be incinerated or treated with a strong basic solution and then autoclaved to ensure complete sterilization.
In addition to surface cleaning, maintenance of equipment such as the air conditioning system must be conducted regularly. This involves comprehensive cleaning and implementation of a routine maintenance program to prevent the growth of biofilms that are notorious for harboring and disseminating pathogens. Therefore, it's not just the visible surfaces that require attention, but also the integral systems that contribute to the overall sanitation and health safety of the facility.