Final answer:
To perform a blood spot collection for a NBS test, you will need a test card, a patient serum sample, and a bottle of test reagent. Using forceps sterilized in alcohol, soak a sterile filter paper blank disk in the positive control solution and place it on three plates.
Step-by-step explanation:
To perform a blood spot collection for a NBS (Newborn Screening) test, you will need to obtain a test card, a patient serum sample, and a bottle of test "reagent" (a suspension of horse erythrocytes) from your instructor. Using forceps sterilized in alcohol, remove a sterile filter paper blank disk from its container and soak it in the positive control solution. Place the soaked disk in the center of the appropriately labeled section on one of the plates, and then repeat the process for the other two plates. This blood spot collection method allows for the analysis of blood samples for various disorders in newborns.