Final answer:
CLSI guidelines suggest holding tubes at an angle and flaming openings during transfers to prevent contamination. Specimens may be frozen at -70 °C or lower for long-term transport, emphasizing aseptic technique during laboratory practices.
Step-by-step explanation:
According to the Clinical and Laboratory Standards Institute (CLSI), tubes such as culture tubes and sample bottles should be transported with proper aseptic techniques to ensure sample integrity and prevent contamination. During the transfer process, tubes or bottles should be held at an angle to minimize the entry of airborne contaminants. Additionally, it is advisable to flame the opening of the tubes or bottles before and after transferring liquids to ward off contaminants.
For the transport of bacteria cultures or medical specimens that require long-term storage, the samples are often frozen at ultra-low temperatures (-70 °C) using dry ice or liquid nitrogen tanks. Specimens intended for transport assays involving sensitive procedures, like those with T. b. brucei and adenosine uptake, would be considerably flash-frozen to preserve their state for further analysis.
In laboratories, practice with sterile water is often recommended before handling live cultures, using tools like pipettes and pipump aids, to ensure aseptic techniques. During practical sessions, cultures are inoculated onto TSA plates or slants and then incubated at specific temperatures to promote bacterial growth.