Question

Which type of light microscope would allow the observer to see a 3D image of an opaque object?

Option 1: Bright-field microscope
Option 2: Phase-contrast microscope
Option 3: Differential interference contrast (DIC) microscope
Option 4: Confocal microscope

Answer 1

The Differential Interference Contrast (DIC) microscope is the type of light microscope that can provide a 3D image of an opaque object.

Step-by-step explanation:

The type of light microscope that allows the observer to see a 3D image of an opaque object is the Differential Interference Contrast (DIC) microscope. This microscope enhances contrast in transparent specimens and creates a pseudo-3D effect by using interference patterns. Bright-field microscopes produce dark images on a bright background and are not ideal for 3D imaging. Phase-contrast microscopes enhance contrast in transparent specimens without staining, and confocal microscopes use laser light to create sharp, optical sections of specimens. However, it is the DIC microscope that is specifically known for providing a 3D-like image of specimens.